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You are here: Home Events June 16, 2014: Tetsuya Higashiyama, Institute of Transformative Bio-Molecules, Nagoya University, Japan

June 16, 2014: Tetsuya Higashiyama, Institute of Transformative Bio-Molecules, Nagoya University, Japan

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Talk in the Biologic Colloquium: "Intercellular Communication and Key Molecules in Pollen Tube Guidance and Early Embryogenesis"

What
  • Biologisches Kolloquium
When Jun 16, 2014
from 04:15 PM to 05:30 PM
Where Lecture Hall Zoology, Hauptstr. 1
Contact Name
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Abstract:

Sexual plant reproduction involves complex cell-to-cell communication. It is, however, difficult to study in the living material due to an embedded structure of female reproductive cells. We have been working on pollen tube guidance, double fertilization, and early embryogenesis in the living material by using two model plant species, Torenia fournieri and Arabidopsis thaliana. Defensin-like peptide LUREs are pollen tube attractants of these species working in a short distance (a few hundred micrometers), which are secreted by two synergid cells on the side of the egg cell (Higashiyama et al., 2001, Science; Okuda et al., 2009, Nature; Takeuchi and Higashiyama, 2012, PLoS Biol.). To understand the molecular mechanism of pollen tube guidance, we have been taking two approaches of live-cell study (for review, Kurihara et al., 2013, Cell Growth Differ.). The first approach is to use precisely defined in vitro system, including development of various microfluidics devices by our engineering team (e.g., Park et al., 2013, Sensors & Actuators B: Chemical; Horade et al., 2013, RSC Adv., 2014, Microelect. Eng.). Recent in vitro studies lead to discovery of novel intercellular signaling molecules involved in competency control of pollen tubes (AMOR glycan), perception of LURE, and long-distance attraction. The second approach is based on in vivo imaging. We have shown that pollen tube guidance is intimately related with double fertilization (Hamamura et al., 2011, Curr. Biol.; Kasahara et al., 2012, Curr. Biol.; Maruyama et al., 2013, Dev. Cell). By using two-photon microscopy, we have succeeded in visualizing pollen tube guidance in the pistil tissue of Arabidopsis. In this seminar, our new live-cell analysis of early embryogenesis in Arabidopsis will also be introduced. By laser disruption, we discovered interesting cell fate conversion in early embryogenesis.

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